Eiichi SOEDA[1]
De-Xing HOU[1]
Tetsusi Yamagata[1]
Haruo KISHIDA[1]
Takanori SHIMOKAWA[1]
Hiroshi FURUHASHI[1]
Emiko SOEDA[1]
Yasuo ATSUCHI[2]
[1]RIKEN Tsukuba
[2]Tokyo Univ.
To construct the physical map of the human chromosome 21 (HC21) with ordered sets of cosmid clones (contig), we have introduced a HC21 specific arrayed library from LLNL and assembled the clones by the fingerprinting method of Carrano et. al. (1) Combination of three commercial available machines, Plasmid DNA Isolator (PI-100, Kurabo), Chemical Robot (DSP-240, Seiko) and Fluorescent Sequencer (DNA sequencer 373A, ABI) have enabled us to analyze a large number of cosmid clones. The software package (Contig Mapper 680A. ABI) has assembled the overlapping clones into contigs which will be mapped by dot blotting analysis with Alu-PCR products of HC21-hybrid cell panel. The dot scorer developed in collaboration with Hitachi SK recorded signals on high-density filters and assigned them to the arrayed library that was able to map them according to the panel. The mapped clones will provide starting materials to be sequenced.